James Watson told an Old Bailey juror today: “I did not kill Rikki Neave”.
His comment came as his lawyer asked him if he had ever had “a sexual interest” in children.
Watson, 40, was 13 at the time and his DNA was found on discarded clothes more than 20 years later.
Watson has claimed he picked up Rikki during a brief encounter in the street with the little boy on the day of his disappearance.
His lawyer Jennifer Dempster QC asked him: "Have you ever had sexual interest in children?"
Watson said: "No, never."
Ms Dempster went on: "Did you have anything to do with the murder of Rikki Neave?"
Watson told jurors: "I wish I could look every one of you 12 times in the eyes. But no, I did not kill Rikki Neave, I did not have anything to do with his disappearance."
Under cross-examination, Watson was asked about an apparent admission he made to police in 2016 that he sexually assaulted a five-year-old boy a year before Rikki's murder.
In 1993, the child had described to police how he and then 12-year-old Watson had touched each other's genitals.
In 2016, Watson dismissed it as "just two boys playing with each other's penises", but in his evidence he claimed to have felt "interrogated".
John Price QC, prosecuting, said: "You are trying to wriggle out of what you admitted to be true six years ago."
Watson replied: "No. I'm saying to you I'm confused what it is you are asking me. I'm not denying anything."
Mr Price continued: "Because bearing in mind the killer of Rikki Neave stripped his dead body naked and then left him posed, you want to get out of the fact the previous year you had sexually assaulted a child of exactly the same age as Rikki."
Watson rejected the comparison, but accepted "something happened" between him and the five-year-old boy.
The defendant was quizzed on his claim that his 2016 account of picking Rikki up to look through a knot hole in a fence was more accurate than his initial account days after Rikki's murder.
Mr Price said: "May I suggest why you have commended the much later account is because you perceived it as necessary to explain why your DNA was found on fibres of Rikki's clothes."
The defendant agreed the theory "fits perfectly", but said that was not his intention.
Watson had earlier spoken about January 2009, when he broke into a British Transport police station and set fire to it.
He said: "I was very angry at the police; the police station bore the brunt of that."
After rifling through the police station, he stole cuffs and batons and after setting the police station alight, made off on a bicycle.
He was also quizzed about a 2018 conviction for sexual assault on a man who had stayed over.
Watson said: "He was effectively sleeping beside me.
“During the night I reached out my hand, it landed on his stomach and briefly - incredibly briefly - my hand touched his penis. I withdrew it straightaway.
"The next morning, I was disgusted with myself."
In August 2005, Watson stole uniform items, equipment and car keys from a police station and left in an unmarked car.
Asked to explain it, he said that "a lot" of his crimes had been to get arrested when his life was not going well.
"I went to a police station - that is the only place I have been where people ask: 'Are you OK? Are you Alright?"'
His "warped view" of police was formed from his experience of his father and being taken into care, jurors heard.
"It was the police that took me away so I was very mixed in my emotions in loving the police, but also hating them," Watson said.
Watson's record also included a number of thefts and burglaries.
He said: "There was a lot of breaking into sheds.
"There was a number of burglaries, a lot of cars.
"When I was in the children's home I would go out with other children and steal a car and set fire to it. It was fun at the time."
With one exception, he had always previously admitted the offences.
He said: "I wanted them to know it was me. Some of the crime I have done was to get in trouble. I have never lied to the police."
Watson is on trial at the Old Bailey accused of strangling Rikki Neave in 1994 and leaving his naked body posed in woodland in Peterborough.
Watson was living at a children's home at the time of Rikki's death and was regarded by social services as a "vulnerable" child.
He could not stay at home after his father, who had previously been a Cambridgeshire Police officer, was arrested and jailed.
While at a children's home in January 1995, Watson stole track signal detonators that gave off a loud noise, and laid them on a railway line.
Watson said: "It was fun. It reads worse than I remember it."
Jurors have heard Watson was on police bail over Rikki's murder in 2016 and living in a hostel as he awaited a charge decision.
In June 2016, he travelled by ferry from Dover to the continent, ending up in Portugal in August of that year before returning to the UK.
Watson said: "I left the country because of the stress I was under.
"I did not know my immediate future; I had been in a hostel for months and months. I wanted some control back.
"My intention was always to come back and answer to my police bail."
Watson, of no fixed address, denies murder and the trial
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